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cy sc007 software fiji
Cy Sc007 Software Fiji, supplied by Spirochrome, used in various techniques. Bioz Stars score: 97/100, based on 907 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cy+sc007+software+fiji/SiR-DNA/pm41145915-160-176-174
Average 97 stars, based on 907 article reviews
Cy Sc007 Software Fiji, supplied by Spirochrome, used in various techniques. Bioz Stars score: 97/100, based on 907 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cy+sc007+software+fiji/SiR-DNA/pm41145915-160-176-174
Average 97 stars, based on 907 article reviews
cy sc007 software fiji - by Bioz Stars,
2026-09
97/100 stars
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Recombinant:Article Title: Nucleosome interaction of the CPC secures centromeric chromatin integrity and chromosome segregation fidelity. Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models INCENP-mAID (RPE1) Hirota Lab 3xFLAG-INCENP Wt_BorealinmNeonGreen (RPE1) Hirota Lab 3xFLAG-INCENP 6A_BorealinmNeonGreen (RPE1) Hirota Lab Borealin-mAID-mClover (RPE1) Hirota Lab Borealin-mCherry Wt (RPE1) Hirota Lab Borealin-mCherry ΔN (RPE1) Hirota Lab Borealin-mCherry 5A (RPE1) Hirota Lab Recombinant DNA Antibodies Mouse anti-Aurora B (WB 1:500) BD Biosciences Cat. 611083 Rabbit anti-INCENP (WB 1:5000) Proteintech Cat. 29419-1-AP Mouse anti-Borealin (WB 1:1000) MBL Cat. M147-3 Mouse anti-Tubulin (WB 1:5000) Millipore Cat. T6074 Rabbit anti-mCherry (WB 1:1000) Gene Tex Cat. GTX128508 Mouse anti-FLAG (WB 1:5000, IF:10000) Sigma-Aldrich Cat. F1804 Mouse anti-CENP-A (IF 1:500) MBL Cat. 3-19 Rabbit anti-CENP-B (IF1:1000) Active motif Cat. 61287 Oligonucleotides and other sequence-based reagents Primers for alpha-satellite Chan et al, 2012 Chemicals, enzymes, and other reagents Micrococcal Nuclease Sigma-Aldrich N3755-50UN Micrococcal Nuclease NEB NEB M0247S Proteinase K Fujifilm 161-28701 RNase A Invitrogen 12091021 Reagent/resource Reference or source Identifier or catalog number Monastrol TOCRIS 1305 Nocodazole Selleck S2775 Palbociclib Selleck S1116 5-Ph-IAA MedChemExpress HY-141894 Puromycin ThermoFisher A1113802 G418 ThermoFisher 11811031 Zeocin Invitrogen R25001 SiR-DNA Spirochrome Cat. CY-SC007 Software Fiji (2.1.0/1.53c) cryoSPARC v4.7.0 RELION 5.0 ChimeraX 1.9 PyMol 3.1 Illustrator Prism 10 Adobe Photoshop 2025 Other Protein expression and purification of CPC constructs The vectors used to express and purify CPC were: pMCNcsINCENP1-58 for untagged INCENP1-58, pEC-S-CDF-His INCENP for His-tagged INCENP1-190 and His-tagged INCENP1-190 6A, pRSET-GFP-Survivin for GFP-tagged full length Survivin and pETM-K-TEV-His-Borealin (pETM vector, gift from C. Romier, Institute of Genetics and Molecular and Cell Biology, Strasbourg, France) for His-tagged Borealin full length, BorealinΔN, BorealinΔN K12A, Borealin3A, BorealinΔN K12A 3A, Borealin5A, BorealinΔN K12A 5A, and BorealinΔloop. Western Blot:Article Title: Nucleosome interaction of the CPC secures centromeric chromatin integrity and chromosome segregation fidelity. Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models INCENP-mAID (RPE1) Hirota Lab 3xFLAG-INCENP Wt_BorealinmNeonGreen (RPE1) Hirota Lab 3xFLAG-INCENP 6A_BorealinmNeonGreen (RPE1) Hirota Lab Borealin-mAID-mClover (RPE1) Hirota Lab Borealin-mCherry Wt (RPE1) Hirota Lab Borealin-mCherry ΔN (RPE1) Hirota Lab Borealin-mCherry 5A (RPE1) Hirota Lab Recombinant DNA Antibodies Mouse anti-Aurora B (WB 1:500) BD Biosciences Cat. 611083 Rabbit anti-INCENP (WB 1:5000) Proteintech Cat. 29419-1-AP Mouse anti-Borealin (WB 1:1000) MBL Cat. M147-3 Mouse anti-Tubulin (WB 1:5000) Millipore Cat. T6074 Rabbit anti-mCherry (WB 1:1000) Gene Tex Cat. GTX128508 Mouse anti-FLAG (WB 1:5000, IF:10000) Sigma-Aldrich Cat. F1804 Mouse anti-CENP-A (IF 1:500) MBL Cat. 3-19 Rabbit anti-CENP-B (IF1:1000) Active motif Cat. 61287 Oligonucleotides and other sequence-based reagents Primers for alpha-satellite Chan et al, 2012 Chemicals, enzymes, and other reagents Micrococcal Nuclease Sigma-Aldrich N3755-50UN Micrococcal Nuclease NEB NEB M0247S Proteinase K Fujifilm 161-28701 RNase A Invitrogen 12091021 Reagent/resource Reference or source Identifier or catalog number Monastrol TOCRIS 1305 Nocodazole Selleck S2775 Palbociclib Selleck S1116 5-Ph-IAA MedChemExpress HY-141894 Puromycin ThermoFisher A1113802 G418 ThermoFisher 11811031 Zeocin Invitrogen R25001 SiR-DNA Spirochrome Cat. CY-SC007 Software Fiji (2.1.0/1.53c) cryoSPARC v4.7.0 RELION 5.0 ChimeraX 1.9 PyMol 3.1 Illustrator Prism 10 Adobe Photoshop 2025 Other Protein expression and purification of CPC constructs The vectors used to express and purify CPC were: pMCNcsINCENP1-58 for untagged INCENP1-58, pEC-S-CDF-His INCENP for His-tagged INCENP1-190 and His-tagged INCENP1-190 6A, pRSET-GFP-Survivin for GFP-tagged full length Survivin and pETM-K-TEV-His-Borealin (pETM vector, gift from C. Romier, Institute of Genetics and Molecular and Cell Biology, Strasbourg, France) for His-tagged Borealin full length, BorealinΔN, BorealinΔN K12A, Borealin3A, BorealinΔN K12A 3A, Borealin5A, BorealinΔN K12A 5A, and BorealinΔloop. Sequencing:Article Title: Nucleosome interaction of the CPC secures centromeric chromatin integrity and chromosome segregation fidelity. Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models INCENP-mAID (RPE1) Hirota Lab 3xFLAG-INCENP Wt_BorealinmNeonGreen (RPE1) Hirota Lab 3xFLAG-INCENP 6A_BorealinmNeonGreen (RPE1) Hirota Lab Borealin-mAID-mClover (RPE1) Hirota Lab Borealin-mCherry Wt (RPE1) Hirota Lab Borealin-mCherry ΔN (RPE1) Hirota Lab Borealin-mCherry 5A (RPE1) Hirota Lab Recombinant DNA Antibodies Mouse anti-Aurora B (WB 1:500) BD Biosciences Cat. 611083 Rabbit anti-INCENP (WB 1:5000) Proteintech Cat. 29419-1-AP Mouse anti-Borealin (WB 1:1000) MBL Cat. M147-3 Mouse anti-Tubulin (WB 1:5000) Millipore Cat. T6074 Rabbit anti-mCherry (WB 1:1000) Gene Tex Cat. GTX128508 Mouse anti-FLAG (WB 1:5000, IF:10000) Sigma-Aldrich Cat. F1804 Mouse anti-CENP-A (IF 1:500) MBL Cat. 3-19 Rabbit anti-CENP-B (IF1:1000) Active motif Cat. 61287 Oligonucleotides and other sequence-based reagents Primers for alpha-satellite Chan et al, 2012 Chemicals, enzymes, and other reagents Micrococcal Nuclease Sigma-Aldrich N3755-50UN Micrococcal Nuclease NEB NEB M0247S Proteinase K Fujifilm 161-28701 RNase A Invitrogen 12091021 Reagent/resource Reference or source Identifier or catalog number Monastrol TOCRIS 1305 Nocodazole Selleck S2775 Palbociclib Selleck S1116 5-Ph-IAA MedChemExpress HY-141894 Puromycin ThermoFisher A1113802 G418 ThermoFisher 11811031 Zeocin Invitrogen R25001 SiR-DNA Spirochrome Cat. CY-SC007 Software Fiji (2.1.0/1.53c) cryoSPARC v4.7.0 RELION 5.0 ChimeraX 1.9 PyMol 3.1 Illustrator Prism 10 Adobe Photoshop 2025 Other Protein expression and purification of CPC constructs The vectors used to express and purify CPC were: pMCNcsINCENP1-58 for untagged INCENP1-58, pEC-S-CDF-His INCENP for His-tagged INCENP1-190 and His-tagged INCENP1-190 6A, pRSET-GFP-Survivin for GFP-tagged full length Survivin and pETM-K-TEV-His-Borealin (pETM vector, gift from C. Romier, Institute of Genetics and Molecular and Cell Biology, Strasbourg, France) for His-tagged Borealin full length, BorealinΔN, BorealinΔN K12A, Borealin3A, BorealinΔN K12A 3A, Borealin5A, BorealinΔN K12A 5A, and BorealinΔloop. Software:Article Title: Nucleosome interaction of the CPC secures centromeric chromatin integrity and chromosome segregation fidelity. Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models INCENP-mAID (RPE1) Hirota Lab 3xFLAG-INCENP Wt_BorealinmNeonGreen (RPE1) Hirota Lab 3xFLAG-INCENP 6A_BorealinmNeonGreen (RPE1) Hirota Lab Borealin-mAID-mClover (RPE1) Hirota Lab Borealin-mCherry Wt (RPE1) Hirota Lab Borealin-mCherry ΔN (RPE1) Hirota Lab Borealin-mCherry 5A (RPE1) Hirota Lab Recombinant DNA Antibodies Mouse anti-Aurora B (WB 1:500) BD Biosciences Cat. 611083 Rabbit anti-INCENP (WB 1:5000) Proteintech Cat. 29419-1-AP Mouse anti-Borealin (WB 1:1000) MBL Cat. M147-3 Mouse anti-Tubulin (WB 1:5000) Millipore Cat. T6074 Rabbit anti-mCherry (WB 1:1000) Gene Tex Cat. GTX128508 Mouse anti-FLAG (WB 1:5000, IF:10000) Sigma-Aldrich Cat. F1804 Mouse anti-CENP-A (IF 1:500) MBL Cat. 3-19 Rabbit anti-CENP-B (IF1:1000) Active motif Cat. 61287 Oligonucleotides and other sequence-based reagents Primers for alpha-satellite Chan et al, 2012 Chemicals, enzymes, and other reagents Micrococcal Nuclease Sigma-Aldrich N3755-50UN Micrococcal Nuclease NEB NEB M0247S Proteinase K Fujifilm 161-28701 RNase A Invitrogen 12091021 Reagent/resource Reference or source Identifier or catalog number Monastrol TOCRIS 1305 Nocodazole Selleck S2775 Palbociclib Selleck S1116 5-Ph-IAA MedChemExpress HY-141894 Puromycin ThermoFisher A1113802 G418 ThermoFisher 11811031 Zeocin Invitrogen R25001 SiR-DNA Spirochrome Cat. CY-SC007 Software Fiji (2.1.0/1.53c) cryoSPARC v4.7.0 RELION 5.0 ChimeraX 1.9 PyMol 3.1 Illustrator Prism 10 Adobe Photoshop 2025 Other Protein expression and purification of CPC constructs The vectors used to express and purify CPC were: pMCNcsINCENP1-58 for untagged INCENP1-58, pEC-S-CDF-His INCENP for His-tagged INCENP1-190 and His-tagged INCENP1-190 6A, pRSET-GFP-Survivin for GFP-tagged full length Survivin and pETM-K-TEV-His-Borealin (pETM vector, gift from C. Romier, Institute of Genetics and Molecular and Cell Biology, Strasbourg, France) for His-tagged Borealin full length, BorealinΔN, BorealinΔN K12A, Borealin3A, BorealinΔN K12A 3A, Borealin5A, BorealinΔN K12A 5A, and BorealinΔloop. Expressing:Article Title: Nucleosome interaction of the CPC secures centromeric chromatin integrity and chromosome segregation fidelity. Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models INCENP-mAID (RPE1) Hirota Lab 3xFLAG-INCENP Wt_BorealinmNeonGreen (RPE1) Hirota Lab 3xFLAG-INCENP 6A_BorealinmNeonGreen (RPE1) Hirota Lab Borealin-mAID-mClover (RPE1) Hirota Lab Borealin-mCherry Wt (RPE1) Hirota Lab Borealin-mCherry ΔN (RPE1) Hirota Lab Borealin-mCherry 5A (RPE1) Hirota Lab Recombinant DNA Antibodies Mouse anti-Aurora B (WB 1:500) BD Biosciences Cat. 611083 Rabbit anti-INCENP (WB 1:5000) Proteintech Cat. 29419-1-AP Mouse anti-Borealin (WB 1:1000) MBL Cat. M147-3 Mouse anti-Tubulin (WB 1:5000) Millipore Cat. T6074 Rabbit anti-mCherry (WB 1:1000) Gene Tex Cat. GTX128508 Mouse anti-FLAG (WB 1:5000, IF:10000) Sigma-Aldrich Cat. F1804 Mouse anti-CENP-A (IF 1:500) MBL Cat. 3-19 Rabbit anti-CENP-B (IF1:1000) Active motif Cat. 61287 Oligonucleotides and other sequence-based reagents Primers for alpha-satellite Chan et al, 2012 Chemicals, enzymes, and other reagents Micrococcal Nuclease Sigma-Aldrich N3755-50UN Micrococcal Nuclease NEB NEB M0247S Proteinase K Fujifilm 161-28701 RNase A Invitrogen 12091021 Reagent/resource Reference or source Identifier or catalog number Monastrol TOCRIS 1305 Nocodazole Selleck S2775 Palbociclib Selleck S1116 5-Ph-IAA MedChemExpress HY-141894 Puromycin ThermoFisher A1113802 G418 ThermoFisher 11811031 Zeocin Invitrogen R25001 SiR-DNA Spirochrome Cat. CY-SC007 Software Fiji (2.1.0/1.53c) cryoSPARC v4.7.0 RELION 5.0 ChimeraX 1.9 PyMol 3.1 Illustrator Prism 10 Adobe Photoshop 2025 Other Protein expression and purification of CPC constructs The vectors used to express and purify CPC were: pMCNcsINCENP1-58 for untagged INCENP1-58, pEC-S-CDF-His INCENP for His-tagged INCENP1-190 and His-tagged INCENP1-190 6A, pRSET-GFP-Survivin for GFP-tagged full length Survivin and pETM-K-TEV-His-Borealin (pETM vector, gift from C. Romier, Institute of Genetics and Molecular and Cell Biology, Strasbourg, France) for His-tagged Borealin full length, BorealinΔN, BorealinΔN K12A, Borealin3A, BorealinΔN K12A 3A, Borealin5A, BorealinΔN K12A 5A, and BorealinΔloop. Purification:Article Title: Nucleosome interaction of the CPC secures centromeric chromatin integrity and chromosome segregation fidelity. Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models INCENP-mAID (RPE1) Hirota Lab 3xFLAG-INCENP Wt_BorealinmNeonGreen (RPE1) Hirota Lab 3xFLAG-INCENP 6A_BorealinmNeonGreen (RPE1) Hirota Lab Borealin-mAID-mClover (RPE1) Hirota Lab Borealin-mCherry Wt (RPE1) Hirota Lab Borealin-mCherry ΔN (RPE1) Hirota Lab Borealin-mCherry 5A (RPE1) Hirota Lab Recombinant DNA Antibodies Mouse anti-Aurora B (WB 1:500) BD Biosciences Cat. 611083 Rabbit anti-INCENP (WB 1:5000) Proteintech Cat. 29419-1-AP Mouse anti-Borealin (WB 1:1000) MBL Cat. M147-3 Mouse anti-Tubulin (WB 1:5000) Millipore Cat. T6074 Rabbit anti-mCherry (WB 1:1000) Gene Tex Cat. GTX128508 Mouse anti-FLAG (WB 1:5000, IF:10000) Sigma-Aldrich Cat. F1804 Mouse anti-CENP-A (IF 1:500) MBL Cat. 3-19 Rabbit anti-CENP-B (IF1:1000) Active motif Cat. 61287 Oligonucleotides and other sequence-based reagents Primers for alpha-satellite Chan et al, 2012 Chemicals, enzymes, and other reagents Micrococcal Nuclease Sigma-Aldrich N3755-50UN Micrococcal Nuclease NEB NEB M0247S Proteinase K Fujifilm 161-28701 RNase A Invitrogen 12091021 Reagent/resource Reference or source Identifier or catalog number Monastrol TOCRIS 1305 Nocodazole Selleck S2775 Palbociclib Selleck S1116 5-Ph-IAA MedChemExpress HY-141894 Puromycin ThermoFisher A1113802 G418 ThermoFisher 11811031 Zeocin Invitrogen R25001 SiR-DNA Spirochrome Cat. CY-SC007 Software Fiji (2.1.0/1.53c) cryoSPARC v4.7.0 RELION 5.0 ChimeraX 1.9 PyMol 3.1 Illustrator Prism 10 Adobe Photoshop 2025 Other Protein expression and purification of CPC constructs The vectors used to express and purify CPC were: pMCNcsINCENP1-58 for untagged INCENP1-58, pEC-S-CDF-His INCENP for His-tagged INCENP1-190 and His-tagged INCENP1-190 6A, pRSET-GFP-Survivin for GFP-tagged full length Survivin and pETM-K-TEV-His-Borealin (pETM vector, gift from C. Romier, Institute of Genetics and Molecular and Cell Biology, Strasbourg, France) for His-tagged Borealin full length, BorealinΔN, BorealinΔN K12A, Borealin3A, BorealinΔN K12A 3A, Borealin5A, BorealinΔN K12A 5A, and BorealinΔloop. Construct:Article Title: Nucleosome interaction of the CPC secures centromeric chromatin integrity and chromosome segregation fidelity. Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models INCENP-mAID (RPE1) Hirota Lab 3xFLAG-INCENP Wt_BorealinmNeonGreen (RPE1) Hirota Lab 3xFLAG-INCENP 6A_BorealinmNeonGreen (RPE1) Hirota Lab Borealin-mAID-mClover (RPE1) Hirota Lab Borealin-mCherry Wt (RPE1) Hirota Lab Borealin-mCherry ΔN (RPE1) Hirota Lab Borealin-mCherry 5A (RPE1) Hirota Lab Recombinant DNA Antibodies Mouse anti-Aurora B (WB 1:500) BD Biosciences Cat. 611083 Rabbit anti-INCENP (WB 1:5000) Proteintech Cat. 29419-1-AP Mouse anti-Borealin (WB 1:1000) MBL Cat. M147-3 Mouse anti-Tubulin (WB 1:5000) Millipore Cat. T6074 Rabbit anti-mCherry (WB 1:1000) Gene Tex Cat. GTX128508 Mouse anti-FLAG (WB 1:5000, IF:10000) Sigma-Aldrich Cat. F1804 Mouse anti-CENP-A (IF 1:500) MBL Cat. 3-19 Rabbit anti-CENP-B (IF1:1000) Active motif Cat. 61287 Oligonucleotides and other sequence-based reagents Primers for alpha-satellite Chan et al, 2012 Chemicals, enzymes, and other reagents Micrococcal Nuclease Sigma-Aldrich N3755-50UN Micrococcal Nuclease NEB NEB M0247S Proteinase K Fujifilm 161-28701 RNase A Invitrogen 12091021 Reagent/resource Reference or source Identifier or catalog number Monastrol TOCRIS 1305 Nocodazole Selleck S2775 Palbociclib Selleck S1116 5-Ph-IAA MedChemExpress HY-141894 Puromycin ThermoFisher A1113802 G418 ThermoFisher 11811031 Zeocin Invitrogen R25001 SiR-DNA Spirochrome Cat. CY-SC007 Software Fiji (2.1.0/1.53c) cryoSPARC v4.7.0 RELION 5.0 ChimeraX 1.9 PyMol 3.1 Illustrator Prism 10 Adobe Photoshop 2025 Other Protein expression and purification of CPC constructs The vectors used to express and purify CPC were: pMCNcsINCENP1-58 for untagged INCENP1-58, pEC-S-CDF-His INCENP for His-tagged INCENP1-190 and His-tagged INCENP1-190 6A, pRSET-GFP-Survivin for GFP-tagged full length Survivin and pETM-K-TEV-His-Borealin (pETM vector, gift from C. Romier, Institute of Genetics and Molecular and Cell Biology, Strasbourg, France) for His-tagged Borealin full length, BorealinΔN, BorealinΔN K12A, Borealin3A, BorealinΔN K12A 3A, Borealin5A, BorealinΔN K12A 5A, and BorealinΔloop. |